Liposomal Encapsulation Services: siRNA

siRNAs have the potential for sequence-specific targeting and inhibition of protein translation. Liposomes composed of amphiphilic lipids, including phospholipids, can trap hydrophilic or hydrophobic materials in hydrophilic inner or hydrophobic lipid bilayers, respectively. The encapsulation process protects the siRNA from degradation, enhances its stability, and enables targeted delivery to specific cells or tissues.

Fig 1. A schematic design of liposomes encapsulating siRNA. (Am Hong et al., 2014)

BOC Sciences' siRNA Liposome Encapsulation Services

BOC Sciences offers a comprehensive range of siRNA liposome encapsulation services based on technical expertise and extensive experience, providing efficient and customizable solutions for encapsulating siRNA molecules to meet their specific needs.

• Formulation Development for siRNA Encapsulation in Liposomes

BOC Sciences performs custom formulation development for siRNA encapsulation in liposomes, including the selection of suitable lipids, optimization of the loaded siRNA, and incorporation of suitable targeting ligands.

• Characterization of siRNA Encapsulation

The liposomes after siRNA encapsulation need to be characterized for physical and chemical properties, including particle size, zeta potential, morphology, and release kinetics of siRNA.

• Evaluation of Efficiency of siRNA Encapsulation

Evaluation of the efficiency of siRNA encapsulation requires cutting-edge technology. BOC Sciences can provide professional technology and instrumentation to test the content and efficiency of siRNA encapsulated in liposomes to ensure that our encapsulation work is efficient.

• Stability assessment of siRNA encapsulation

Liposomes encapsulated with siRNA need to be physically and chemically stable when they are made into formulations. We provide rigorous stability studies to ensure that liposome formulations are suitable for transportation and long term storage.

• Quality Control

BOC Sciences maintains stringent quality control measures throughout the encapsulation process to ensure consistent and reliable performance of its liposome-encapsulated siRNA formulations.

Liposome-encapsulated siRNA Applications

Cancer therapy: Liposome-encapsulated siRNAs can be designed to target oncogenes or oncogenes to selectively silence genes involved in cancer development and progression.

Inflammatory diseases: By targeting specific inflammatory mediators, liposome-encapsulated siRNAs can help reduce inflammation associated with diseases such as rheumatoid arthritis and inflammatory bowel disease.

Genetic Diseases: Liposomal encapsulation of siRNAs has the potential to treat genetic diseases by selectively silencing disease-causing genes or correcting aberrant gene expression.

Viral infections: Liposomal encapsulation of siRNAs can be used to target viral genes and inhibit viral replication, providing a potential therapeutic strategy for viral infections.

Neurological disorders: Liposome-mediated delivery of siRNAs to the central nervous system holds promise for the treatment of neurodegenerative disorders through selective silencing of disease-associated genes.

Advantages of Liposomal Encapsulation of siRNAs

• Enhanced stability - Liposomal encapsulation protects siRNA from enzymatic degradation and other environmental factors, ensuring stability and integrity during storage and transportation.
• Targeted delivery - Liposomes can be modified with targeted ligands to deliver siRNA specifically to diseased cells or tissues. This targeted approach improves therapeutic efficacy while minimizing off-target effects.
• Prevention of immune response - Liposomes shield siRNAs from immune surveillance, reducing recognition and potential clearance by the immune system, thereby prolonging circulation time in the body.
• Controlled release - Liposomes can be designed to enable controlled and sustained release of siRNA, ensuring prolonged therapeutic effects and reduced dosing frequency.
• Versatility - Liposome encapsulation is compatible with a wide range of cargoes, including siRNAs, small molecules, proteins and nucleic acids, providing a versatile platform for drug delivery.

Reference

1. Am Hong C, Nam Y S. Functional nanostructures for effective delivery of small interfering RNA therapeutics[J]. Theranostics, 2014, 4(12): 1211.