Liposomal Encapsulation Services: Proteins & Peptides

The efficiency of liposomal encapsulation of proteins/peptides depends on the interaction between the protein/peptide and the lipid bilayer. By controlling the lipid composition of liposomes, or by increasing the concentration of liposomes, the encapsulation efficiency of proteins/peptides can be increased to facilitate electrostatic interactions. Currently, advanced platforms and proven technologies have successfully integrated peptides and proteins into liposomes for different drug delivery routes.

Fig 1. Liposome encapsulation of anionic and cationic whey peptides. (Mohan et al., 2018)

Characteristics of Liposome-encapsulated Proteins/Peptides

• Provide sustained release of peptides/proteins and prevent degradation during drug delivery.
• Flexible architecture and composition: formulations can be adjusted.
• Increased tissue permeability of protein/peptide formulations.

Types of Liposomes as Carriers

• Conventional Liposomes

Conventional liposomes can encapsulate hydrophilic proteins and peptides within their aqueous core or hydrophobic molecules within a lipid bilayer.

• Polyethylene Glycol-modified Liposomes

Polyethylene glycol-modified liposomes form a hydrated membrane on the surface of the liposome, providing a large spatial barrier to the liposome and masking the hydrophobic binding sites on the surface.

• Cationic Liposomes

A cationic liposome is a lipid vesicle that is itself positively charged. It can be used as a carrier for negatively charged substances, especially for proteins, peptides and oligonucleotides.

• pH-sensitive Liposomes

pH-sensitive liposomes are functional liposomes with the ability to target and control the release of drugs (e.g. genes, nucleic acids, peptides, proteins) from cells. The principle is that low pH leads to protonation of the carboxyl group of the fatty ester, which causes the formation of a hexagonal crystalline phase, the main mechanism of membrane fusion.

BOC Sciences' Proteins & Peptides Encapsulation Services

BOC Sciences' liposome development platform provides you with the best liposome encapsulation services. We can prepare liposomes by selecting specific phospholipids and technologies based on the encapsulated molecule or release conditions. The optimal choice of liposome encapsulation technology depends on the physicochemical properties of the material to be encapsulated, the size and polydispersity of the vesicles required, the nature of the bilayer required, and the size of the simplified process.

In the service of liposome encapsulation proteins & peptides we offer:

• Targeting design and optimization of liposome-encapsulated proteins & peptides
• Customized Liposomes of various sizes, sheet materials and physicochemical properties
• Complete test reports for liposome testing

Liposome encapsulation offers a promising approach to enhance stability, bioavailability and targeted delivery of proteins and peptides. With our expertise, state-of-the-art facilities, and commitment to quality, BOC Sciences can provide comprehensive liposome encapsulation services tailored to our clients' specific needs. Harnessing the power of liposomes, our goal is to facilitate the development of innovative therapies and advance the field of protein and peptide delivery.

• Factors to consider for proteins and peptides as encapsulated molecules

Structural Integrity: Proteins and peptides are prone to conformational changes and aggregation due to their own structural properties, which can affect the efficiency of their encapsulation in liposomes. To ensure the structural integrity of proteins and peptides, formulations and encapsulation conditions need to be optimized.

Activity and Function: In order to ensure that proteins and peptides retain their activity and function in liposomes, it is necessary to optimize the encapsulation conditions, select the appropriate liposome components, avoid exposure to extreme conditions and store in appropriate conditions.

BOC Sciences provides clients with high-quality liposome encapsulation services. For more information, you can contact us at any time.

Reference

1. Mohan A, et al. Liposome encapsulation of anionic and cationic whey peptides: Influence of peptide net charge on properties of the nanovesicles[J]. Lwt, 2018, 87: 40-46.