Liposomal Encapsulation Services: Labeling Agents

In the process of liposomal encapsulation of labeling agents (e.g., a fluorescent dye, radioisotope, or other molecule), which are typically mixed with the constituent parts of the liposome and encapsulated inside or on the surface of the liposome by an appropriate method. This allows for the protection and stabilization of the labeling agent and allows for the delivery of the labeling agent to the target tissue or cell using the properties of liposomes, such as tunable size, surface modification, and targeting capabilities.

Fig 1. Liposome labeling of highly efficient MRI contrast agents. (Cheng et al., 2014)

Based on BOC Sciences' expertise and equipment, we are able to provide services for encapsulation of labeling agents in liposomes, as well as customized design and implementation of labeling agents encapsulation according to customers' needs.

Services for Encapsulating Labeling Agents in Liposomes

BOC Sciences offers services for encapsulation of labeling agents in liposomes in a step-by-step process that includes:

• Labeling Agents Selection - Labeling agents can be selected according to customer requirements and can be fluorescent dyes, fluorescent proteins, isotopes, and contrast agents.
• Encapsulation of Labeling Agents - Liposomes encapsulated with labeling agents can be prepared using techniques such as membrane solubilization, membrane fusion, and ultrasonic methods, and these labeling agents can be on the inside of the liposomes or on the surface of the liposomes.
• Removal of Unencapsulated Labeling Agents - After the labeling agents have been encapsulated, the unencapsulated labeling agents need to be removed using size exclusion columns, ultrafiltration centrifugation, etc., which in turn improves the encapsulation efficiency and purity.
• Characterization of Encapsulated Liposomes - Liposomes are physically characterized by professional analytical instruments and techniques, including particle size analysis, stability testing, and kinetic analysis of release behavior, to assess encapsulation efficiency and stability.

Types of Labeling Agents Encapsulated in Liposomes

• Fluorescent Dyes

Fluorescent dyes are extremely sensitive and easy to use, and can be encapsulated in liposomes for studying intracellular processes, bio-imaging, and more. Options for fluorescent dyes include fluorescein, rhodamine, and fluorescent yellow.

• Fluorescent Proteins

Fluorescent proteins used for encapsulation are generally green fluorescent proteins, which can be used for cell labeling, protein localization, and other areas.

• Radioisotopes

Liposomes encapsulated with radioisotope labeling agents can be used to track and quantify the distribution and metabolism of liposomes in the body.

• Contrast Agents

Contrast agents can be categorized into two main groups, high-density contrast agents with high atomic weight and high specific gravity, and low-density contrast agents with low atomic weight and low specific gravity. Liposome-encapsulated contrast agents can be used to increase the stability of contrast agents, improve their pharmacokinetics, enhance targeting and reduce side effects. They can be used in medical diagnostics, tumor therapy monitoring and drug delivery.

The following factors need to be considered when selecting labeling agents:

Purpose of Application: The selection of labeling agents should match the purpose of the study. Different types of labeling agents are suitable for different research areas and experimental designs.

Stability: Labeling agents should have good stability and be able to maintain their activity and fluorescence properties during liposome preparation and application.

Compatibility: Labeling agents should be compatible with the liposome components and be able to bind efficiently into the liposome and maintain its activity and function.

The experienced staff at BOC Sciences ensures a high quality liposome manufacturing process and a cost-effective, reliable, and consistently high quality product. At BOC Sciences, we are committed to excellence while providing quality assured products for research and development. If you are interested in our programs, please feel free to contact us.

Reference

1. Cheng Z, et al. Stabilized porous liposomes with encapsulated Gd-labeled dextran as a highly efficient MRI contrast agent[J]. Chemical Communications, 2014, 50(19): 2502-2504.