Polysaccharide-Coated Liposome Synthesis Service

Comprehensive Service for Polysaccharide-Coated Liposome Synthesis

At BOC Sciences, we provide a wide range of tailored services for Polysaccharide-Coated Liposome Synthesis that are highly customizable to meet the specific needs of your research and development projects. Our capabilities span from initial formulation design to advanced analytical characterization, offering flexibility and scientific rigor to help optimize drug delivery, gene transfection, and biomolecular encapsulation.

Step-by-Step Process of Polysaccharide-Coated Liposome Synthesis Service

At BOC Sciences, we follow a precise, multi-step process to ensure the creation of high-quality polysaccharide-coated liposomes tailored to your specific research needs. Our approach integrates cutting-edge technology and expert knowledge to deliver consistent, reproducible results for your preclinical applications.

1. Consultation & Feasibility Assessment

• Initial discussion with clients to understand research goals, liposomal formulation, and encapsulant (e.g., drug, RNA, or protein).
• Detailed evaluation of target applications to select the optimal liposome core and polysaccharide coating.
• Customization of liposomal systems to meet specific needs, such as drug delivery, gene transfection, or targeted therapies.

2. Liposome Contract Manufacturing Services

• Selection of lipid composition (e.g., phosphatidylcholine, cholesterol, DOTAP) based on desired liposome type (SUV, MLV, LUV).
• Custom liposomes are synthesized using methods such as thin-film hydration, microfluidics, or ethanol injection.
• Focus on achieving the appropriate size, encapsulation efficiency, and overall stability of the liposome core.

3. Polysaccharide Activation & Modification

• Activation of polysaccharides (e.g., chitosan, dextran, hyaluronic acid) through chemical processes like EDC/NHS activation or thiolation.
• Modification of polysaccharide molecular weight and substitution degree to optimize for target interaction or mucoadhesion.
• Preparation of polysaccharides for covalent or electrostatic conjugation to the liposome surface.

4. Liposome Targeted Modification

• Polysaccharide coating applied to liposomes via methods like electrostatic adsorption, covalent coupling, or layer-by-layer deposition.
• Tailoring of coating thickness and stability to enhance circulation time, cellular uptake, and targeting capabilities.
• Coating is optimized to meet application-specific requirements, such as receptor-ligand targeting or improved bioavailability.

5. Post-Coating Purification & Quality Control

• Purification of coated liposomes to remove unbound polysaccharides or excess reagents using ultrafiltration or centrifugation.
• Analytical testing to verify particle size, surface charge (zeta potential), encapsulation efficiency, and coating integrity.
• Use of advanced techniques like FTIR and NMR to confirm the chemical structure of the polysaccharide coating.

6. Data Delivery & Support

• Comprehensive stability testing under various conditions (e.g., temperature, pH) to assess the robustness of the liposome formulation.
• Evaluation of release profiles for encapsulated agents to predict behavior in vitro or in vivo.
• Delivery of a detailed report, including formulation data, analytical results, stability metrics, and recommendations for storage and handling.

Benefits of Our Polysaccharide-Coated Liposome Synthesis

Applications of Our Polysaccharide-Coated Liposome Synthesis

Polysaccharide-coated liposomes offer a range of unique advantages, enabling highly specialized applications in preclinical drug delivery, gene therapy, and nanomedicine research.

Targeted Gene Delivery with Polysaccharide-Coated Liposomes

Polysaccharide coatings, particularly chitosan and dextran, enhance the delivery of genetic materials such as siRNA, mRNA, and plasmid DNA by facilitating endosomal escape and improving cellular uptake. These coatings can also be tailored to target specific cell types, such as those expressing CD44 receptors, making them ideal for targeted gene delivery systems.

• Hyaluronic Acid-Coated Liposomes target CD44 receptors overexpressed in tumors, facilitating precise gene delivery.
• Chitosan-Coated Liposomes enhance the stability and uptake of RNA therapeutics.

Enhanced Tumor Targeting via Receptor-Mediated Uptake

Polysaccharide-coated liposomes, especially those modified with hyaluronic acid, dextran, or mannose, are a cornerstone for tumor-targeted drug delivery. These coatings facilitate active targeting by exploiting overexpressed cell surface receptors in tumor cells, such as CD44 (for hyaluronic acid) or mannose receptors (for dextran).

• Hyaluronic Acid-Coated Liposomes are ideal for targeting solid tumors like breast or lung cancer, improving selective internalization of chemotherapeutics.
• Mannose-Dextran-Coated Liposomes target macrophage receptors in the tumor microenvironment, enhancing drug accumulation and efficacy.

Brain-Targeted Drug Delivery for Neurodegenerative Diseases

One of the most exciting areas of application for polysaccharide-coated liposomes is brain-targeted drug delivery. Polysaccharide coatings, particularly hyaluronic acid and dextran, can enhance blood-brain barrier (BBB) penetration, a major challenge in the treatment of central nervous system (CNS) disorders.

• Hyaluronic Acid-Coated Liposomes utilize CD44 receptors to facilitate brain-targeted delivery of neuroprotective drugs or gene therapies for diseases like Alzheimer's.
• Dextran-Coated Liposomes enhance CNS drug delivery, ideal for brain cancer or neurotrophic factor therapies.

Mucosal and Oral Drug Delivery Systems

Polysaccharide-coated liposomes are highly effective in mucosal drug delivery, as they can be designed to enhance bioadhesion to mucosal surfaces in the gastrointestinal (GI) tract or nasal passages. The mucoadhesive properties of polysaccharides like chitosan or alginate allow for sustained drug release and improved absorption of poorly water-soluble drugs or biologics.

• Chitosan-Coated Liposomes are effective for oral delivery of proteins and vaccines, improving intestinal absorption and protecting against enzymatic degradation.
• Alginate-Coated Liposomes enhance drug delivery to the intestinal epithelium, increasing bioavailability for oral formulations.

Controlled and Sustained Release Systems

The use of polysaccharide coatings, such as chitosan or alginate, allows for controlled release of active pharmaceutical ingredients (APIs) from liposomes, providing a sustained release profile that can improve therapeutic efficacy and reduce dosing frequency.

• Chitosan-Coated Liposomes provide long-acting formulations for anticancer drugs, hormonal therapies, or antibiotics, enhancing patient compliance.
• Alginate-Coated Liposomes offer pH-sensitive release, ensuring targeted delivery in the GI tract or for topical treatments.

FAQs – Insights about Our Polysaccharide-Coated Liposome Synthesis Service

What types of polysaccharides can you work with?

We offer a wide variety including chitosan, dextran, hyaluronic acid, alginate, pullulan, and others. We can also functionalize polysaccharides with ligands or reactive groups for custom conjugation.

Can I request a specific polysaccharide-to-lipid ratio in the coating?

Absolutely. We offer customizable surface compositions, including precise control over the polysaccharide-to-lipid ratio. This is critical for tuning zeta potential, cellular uptake, and interaction with biological environments. Our experts will guide you based on your application requirements.

How do polysaccharide-coated liposomes compare to PEGylated liposomes in terms of performance?

Polysaccharide coatings provide a natural, biodegradable alternative to PEG. They can offer enhanced bioadhesion, targeted delivery through receptor-mediated uptake (e.g., HA-CD44), and reduced immunogenicity. If your study requires a biologically interactive or mucosal-adhesive system, polysaccharides may offer superior performance.

Can I request dual coatings or multilayer structures with different polysaccharides?

Yes, we offer multilayer coating strategies, including layer-by-layer assembly using sequential polysaccharides or combinations (e.g., chitosan-alginate, HA-dextran). This is especially valuable for sustained release or multi-targeted delivery systems in preclinical models.

Can you help with polysaccharide-coated liposome formulation for nucleic acid delivery (siRNA, mRNA, etc.)?

Yes. We specialize in cationic liposome cores (e.g., DOTAP-based) with polysaccharide coatings like chitosan or mannose-modified polysaccharides, which enhance cellular uptake and endosomal escape in transfection studies. Coatings can be functionalized with ligands for targeted gene delivery.

Partner with BOC Sciences to accelerate your preclinical research with precision-engineered polysaccharide-coated liposomes. We combine nanotechnology expertise, chemical engineering precision, and client-centric service to bring your formulations to life—reliably and reproducibly.