DODAP Liposomes for DNA/RNA Delivery

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DODAP (or 1,2-dioleoyl-3-dimethylammonium-propane) liposomes are cationic lipids that are an active area of research and development for nucleic acid drug and gene delivery system applications. Based on the importance of DODAP liposomes in DNA and RNA delivery, BOC Science is committed to providing high-quality liposomal products and related customization services to customers with DODAP liposome needs.

What are DODAP Liposomes?

DODAP is an ionizable lipid with low cytotoxicity and high transfection efficiency. DODAP has a positively charged head group that can interact with the negatively charged phosphate backbone of DNA/RNA molecules to form a DODAP-DNA/RNA complex. Therefore, DODAP is very suitable for use as a DNA/RNA delivery vehicle with high stability, safety and biocompatibility, which can be designed for the controlled release of drugs in specific tissues and cells. By stably and efficiently transporting nucleic acid drugs (e.g., siRNAs, ASOs, mRNAs, DNA plasmid, etc.) and gene tools (gene fragments, gene editing tools, etc.), DODAP liposomes have a wide range of promising applications in the field of gene therapy.

Figure 1. The stucture of DODAP liposome.

Advantages of DODAP Liposomal Delivery of DNA/RNA

Easy Preparation of DODAP-DNA/RNA Complexes

DODAP has a positively charged ammonium group that very easily forms lipid-nucleic acid complexes with negatively charged DNA/RNA by electrostatic interaction. This electrostatic interaction promotes the interaction between DODAP and nucleic acid molecules and is a very suitable choice of carrier.

Improved Cellular Uptake and Internalization

As a cationic liposome, DODAP enhances cellular uptake of DODAP-DNA/RNA complexes. Cellular endocytosis is a key step in the delivery of nucleic acids to the target cell, and DODAP enhances the efficiency of this process to promote cellular uptake of nucleic acid drugs and thereby enhance drug efficacy.

Improved Stability of DNA/RNA Transport

The biggest challenge in the transportation of DNA or RNA in the vivo is that it is easily degraded by enzymes, and encapsulation of the transported DNA or RNA is very necessary. DODAP protects encapsulated DNA or RNA from enzymatic degradation in the extracellular environment, thus improving the stability of nucleic acids.

Site-specific Controlled Release

Depending on the design of the DODAP liposome formulation, it is possible to achieve targeted controlled release of encapsulated DNA or RNA for long-term effects. If you have a relevant project requirement, BOC Sciences can assist in the design and optimization of a suitable DODAP liposome formulation based on your needs, taking into account the following aspects.

Type of nucleic acid to be delivered
Nucleic acid payload
Lipid composition fine-tuning design
Safety assessment

Figure 2. Example design for DODAP liposomal delivery of nucleic acids. (S, Kimura.; et al, 2021)

Benefits of BOC Sciences' DODAP Products and Custom Services

BOC Sciences manufactures and supplies a wide range of high quality DODAP products and customized DODAP products, both GMP-grade and non-GMP-grade, to assist in research and development in the areas of nucleic acid drug and gene fragment delivery.

BOC Sciences implements stringent quality control in the manufacturing plant and adheres to optimized and efficient separation and purification strategies to ensure that you get high purity DODAP products.

With high-end production equipment, optimized production processes and high production efficiency, BOC Sciences is committed to providing you with larger quantities and shorter lead times.

BOC Sciences has a team of experts with master's degree or above, and more DODAP lipid-based design and delivery systems are under development to meet different project requirements.

For more custom services about our amine reactive liposomes, please feel free to contact us.

Reference

S, Kimura.; et al. Novel Lipid Combination For Delivery Of Plasmid DNA To Immune Cells In The Spleen. Journal of Controlled Release. 2021, 330: 753-764.