Cationic Liposomes

BOC Sciences provides a series of cationic liposomes for plasmid DNA, antisense, siRNA, etc. delivery.

Our advantages

• Rich product range - providing multi-type liposomes containing various zeta potentials and different characteristics
• A manufacturing process that meets GMP requirements
• High-quality lipid raw materials to ensure the high performance of our product
• All products are subject to strict quality inspection and quality control during the production process
• Experienced liposome experts will provide you with professional technical guidance throughout the process
• Advanced analysis equipment
• The most competitive price in the entire network
• Delivery within 24h (the delivery time of ustomized products need to be determined according to the actual situation)

What is Cationic liposome?

Cationic liposomes are the core components of nanoparticles, with a common structure of a positively charged head group and one or two hydrophobic tail regions made of hydrocarbon chains or steroid structures.

Synthesis method for cationic lipid

• Design based on cholesterol, such as DC-cholesterol and GL-67 lipid
• Design based on non-cholestero, such as DOTAB, DDAB and DOTMA, DOTAP

Different endocytic pathways may be related to the composition of cationic liposomes

• Lipid complexes composed of DC-Chol or DOPE-based cationic liposomes preferentially enter cells through raft-mediated endocytosis.
• Liposomes including 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) or cationic liposomes based on oleoyl phosphocholine are absorbed by non-specific liquid phase giant cell phagocytosis.

The lipid composition of liposomes affects the structural properties and transfection efficiency of cationic lipids

Cationic liposomes composed of 3β-[ N-(N',N'-dimethylaminoethane)carbamoyl]cholesterol (DC-Chol) and DOPE are considered to be representative liposomes that effectively deliver genes . For the delivery of plasmid DNA, the most effective molar ratio of DC-Chol to DOPE was found to be 1:2. The transfection efficiency of plasmid DNA decreases as the weight ratio of DC-Chol to plasmid DNA increases, and the highest efficiency is 3:1.

Figure 1: Strategies for cationic liposome-based nucleic acid delivery (Gayong Shim, 2013).

Advantages of Cationic liposome

Cationic liposomes are used to deliver various nucleic acids, such as plasmid DNA, antisense oligonucleotides, and siRNA. As a nucleic acid delivery system, cationic liposomes have many advantages.

• First, cationic liposomes are biodegradable after in vivo administration. Endogenous enzymes can break down the lipid components of liposomes. The unparalleled biocompatibility of liposomes among various nanocarriers has led to the use of cationic liposomes to deliver various siRNAs for in vivo studies.
• The lipid component's adjustment of surface charge density can control the interaction force with negatively charged nucleic acids.
• The inclusion of pegylated lipids or functional lipids can enable various surface modifications of liposomes.
• In addition, the inclusion of lipophilic chemicals in the lipid bilayer of cationic liposomes can co-deliver anticancer drugs and therapeutic nucleic acids.

References

1. Gayong Shim, Mi-Gyeong Kim, Joo Yeon Park, Yu-Kyoung Oh. Application of cationic liposomes for delivery of nucleic acids. Asian Journal of Pharmaceutical Sciences. 2013; 8(2):72-80.
2. Yinan Zhao, Defu Zhi and Shubiao Zhang. Cationic Liposomes in Different Structural Levels for Gene Delivery. Non-Viral Gene Therapy. 2011:293-318.
3. Carola Hofmann, Barbara Kaiser, Susanne Maerkl, Axel Duerkop & Antje J. Baeumner. Cationic liposomes for generic signal amplification strategies in bioassays. Analytical and Bioanalytical Chemistry. 2020; 412:3383-3393.
4. Jeroen Heuts, etc. Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides. Pharm Res. 2018; 35(11):207.