Azide Reactive Liposomes

Online Inquiry

Azide reactive liposomes are functional liposomes with azide reactive group modifications on the surface that can be conjugated to other chemical entities via click reactions. BOC Sciences is committed to providing you with high quality and competitively priced azide-reactive liposomes designed to use the process of click chemistry to concatenate antibodies to obtain immunoliposomes, advancing drug delivery and development in related fields.

What are Azide Reactive Liposomes?

Azide-reactive liposomes with azide (N3) groups on the surface can react with other molecules or compounds having complementary reactive groups, such as alkyne groups (-C=CH), to form covalent bonds via copper-free click chemistry. This reactivity allows for the attachment of various biomolecules, drugs or targeting ligands (especially antibodies) to the surface of liposomes for specific applications in drug delivery, diagnostics and therapy.

Figure 1. Miscibility of azide-modified membrane lipids with saturated phosphatidylcholine and saturated phosphatidylcholine, respectively. (S, Müller.; et al. 2019)

Azide Functional Groups

Azido groups (-N3) are chemically reactive and can participate in a variety of bioconjugation reactions with high specificity, efficiency and biocompatibility. They are commonly used to link liposomes to other molecules or ligands such as antibodies, peptides, or visualizers. This is a stable and conjugated chemical structure that can play an important role in many biological applications such as biomarkers and drug delivery. Common groups that can click chemically with azide groups are in the following.

Alkyne Group, -C≡C-: The "Azide-Alkyne" click chemistry reaction is one of the most common and widely used click chemistry reactions, where the product formed is a stabilized triazole.

Cyclooctyne Group: The "Azide-Cyclooctyne" click chemistry, which has a lower background reaction relative to the alkyne moiety, can be used for highly selective conjugation in biology.

Copper-free Click Chemistry

Copper-free click chemistry conjugation is a method used to attach material macromolecules to a carrier surface without the use of copper ions as a catalyst. The advantage of this method is not only that it can be performed in a biological environment, avoiding the toxicity of copper ions to biomolecules, but also that it is highly specific and efficient.

Figure 2. Efficient click chemistry coupling reactions of alkyne and DBCO.

Applications of Azide Reactive Liposomes

Azide reactive liposomes are capable of promoting bioconjugation through click chemistry in downstream reactions and have a wide range of applications mainly in drug delivery system research, bioimaging, and targeted drug development.

Drug Delivery System Development

Azide-reactive liposomes can be designed to load nucleic acid drugs, anticancer drugs, enzymes for the development of drug delivery systems.

Targeted Drug Delivery

Azide-reactive liposomes can be functionalized with targeting ligands (e.g., antibodies or peptides) that specifically recognize a particular cell. This allows the corresponding drug to be selectively delivered to a specific site, reducing systemic side effects.

Bioimaging Tools

Azide-reactive liposomes can be loaded with contrast agents or imaging probes with targeted molecular functionality. This enables specific imaging of diseased tissues or cells in vivo, contributing to early disease detection and monitoring of treatment response.

Allowing Design of Multifunctional Modifications

Azide functionalization can be used to design liposomes with stimulus-responsive properties, such as pH-responsive or temperature-sensitive, to enable controlled and triggered drug release at specific sites.

Benifits of BOC Sciences' Azide Reactive Liposomes

High Product Quality is Our First Priority: BOC Sciences has GMP-grade manufacturing facilities, stringent quality control, and optimized isolation and purification strategies to ensure that you get high purity azide-reactive liposomes.

Highly Specific Splicing and Splicing Efficiency: Click chemistry is biologically orthogonal and specific to improve downstream splicing efficiency. azide-reactive liposomes from BOC Sciences are based on click chemistry and excel in antibody adhesion.

Comprehensive Technical Support and After-sales Service: BOC Sciences' experts are dynamic PhDs who ensure expertise and technical support, and are available for consultation on any subsequent use issues.

Customized Liposome Products: BOC Sciences also offers customized azide-responsive liposomes, which can be tailored to the specific modifications, formulations, or loading ratios of the type of liposome required for your project.

For more custom services about our azide reactive liposomes, please feel free to contact us.

Reference

S, Müller.; et al. Azide-Modified Membrane Lipids: Miscibility with Saturated Phosphatidylcholines. Langmuir. 2019, 35(38): 12439-12450.